Detection of Badnavirus in pineapple in northeastern Brazil

Edlene Maria da Silva Moraes Santos; Jaqueline Figueredo de Oliveira Costa; Mayra Machado de Medeiros Ferro; Sarah Jacqueline Cavalcanti da Silva; Iraildes Pereira Assunção; Gaus Silvestre de Andrade Lima

doi:10.17648/diversitas-journal-v5i4-1284

Detection of Badnavirus in pineapple in northeastern Brazil

Authors

Edlene Maria da Silva Moraes Santos Universidade Federal Rural de Pernambuco
Jaqueline Figueredo de Oliveira Costa Universidade Federal de Alagoas/Campus de Engenharias e Ciências Agrárias
Mayra Machado de Medeiros Ferro Universidade Federal de Alagoas/Campus de Engenharias e Ciências Agrárias
Sarah Jacqueline Cavalcanti da Silva Universidade Federal de Alagoas/Campus de Engenharias e Ciências Agrárias
Iraildes Pereira Assunção Universidade Federal de Alagoas/Campus de Engenharias e Ciências Agrárias
Gaus Silvestre de Andrade Lima Universidade Federal de Alagoas/Campus de Engenharias e Ciências Agrárias

DOI:

https://doi.org/10.17648/diversitas-journal-v5i4-1284

Abstract

ABSTRACT: Brazil is one of the main global producers of pineapple (Ananas comosus L. Merril), with emphasis in the Northeastern region of the country. Pineapple is exposed to several phytosanitary problems, including viruses. Pineapple badnaviruses are caused by two distinct species: Pineapple bacilliform CO virus (PBCoV) and Pineapple bacilliform ER virus (PBErV). The present study aimed to detect possible species of the genus Badnavirus in pineapple samples in Northeastern Brazil, via PCR and sequencing of the RT/RNaseH region. Leaf samples of pineapples were collected in the states of Alagoas, Maranhão, Paraiba and Pernambuco, and subsequently subjected to total DNA extraction and amplification via PCR. Badnavirus positive samples were selected for sequencing. Analysis of pairwise comparisons revealed that all sequences obtained in this work showed an identity greater than 80% with the sequence of the species PBCoV (EU377664), from Australia, fully corroborating with phylogenetic analyzes. These results suggest the widespread of PBCoV in Northeastern Brazil and record the first report of Badnavirus in pineapple culture in Brazil.

KEYWORDS: Caulimoviridae, Ananas comosus, molecular analysis.

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Published

2020-10-26

How to Cite

Santos, E. M. da S. M., Figueredo de Oliveira Costa, J., Ferro, M. M. de M., Silva, S. J. C. da, Assunção, I. P., & Lima, G. S. de A. (2020). Detection of Badnavirus in pineapple in northeastern Brazil. Diversitas Journal, 5(4), 2450–2463. https://doi.org/10.17648/diversitas-journal-v5i4-1284

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Issue

Vol. 5 No. 4 (2020): Agricultura familiar: a base da economia latinoamericana

Section

Ciências Agrárias

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Copyright (c) 2020 Edlene Maria da Silva Moraes Santos, Jaqueline Figueredo de Oliveira Costa, Mayra Machado de Medeiros Ferro, Sarah Jacqueline Cavalcanti da Silva, Iraildes Pereira Assunção, Gaus Silvestre de Andrade Lima

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